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Update Genome annotation with Braker3#6979

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tflowers15 wants to merge 3 commits into
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tflowers15:genome-annotate-braker
Open

Update Genome annotation with Braker3#6979
tflowers15 wants to merge 3 commits into
galaxyproject:mainfrom
tflowers15:genome-annotate-braker

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@tflowers15

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I have made some minor updates to the Genome annotation with Braker3 tutorial to correct mismatches between the names of the imported datasets and the input dataset names used in the tool setup steps.

Imported datasets from Zenodo:

  • https://zenodo.org/records/14770765/files/genome.fasta
  • https://zenodo.org/records/14770765/files/RNASeq.bam
  • https://zenodo.org/records/14770765/files/protein_sequences.fasta

Tool input dataset names -> Corresponding imported file from Zenodo

  • genome_masked.fasta -> genome.fasta
  • SwissProt_subset.fasta -> protein_sequences.fasta

Removed instructions for importing datasets from Data Library as the listed Data Library on the usegalaxy.* servers that should contain the datasets for this tutorial does not appear to exist: GTN - Material -> genome-annotation -> Genome annotation with Braker3.

Corrected the tool setup for GFFread to select the protein fasta output dataset (current instructions are to output the exon fasta dataset), which is used as an input for the following BUSCO and OMArk steps (specificed input is GFFread pep.fa).

  • “Select fasta outputs”: protein fasta file with the translation of CDS for each record (-y)

@abretaud

abretaud commented Jul 6, 2026

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thanks! ping @rlibouba

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