diff --git a/packages/vite/src/node/plugins/resolve.ts b/packages/vite/src/node/plugins/resolve.ts
index 7bd7282aed712b..b4ec18d4157192 100644
--- a/packages/vite/src/node/plugins/resolve.ts
+++ b/packages/vite/src/node/plugins/resolve.ts
@@ -450,6 +450,18 @@ function optimizerResolvePlugin(
 
             const normalizedFsPath = normalizePath(fsPath)
 
+            if (asSrc && !options.scan && !SPECIAL_QUERY_RE.test(id)) {
+              const resolvedFsPath = tryFsResolve(fsPath, options)
+              const optimizedDep = depsOptimizer.metadata.depInfoList.find(
+                (depInfo) =>
+                  depInfo.src ===
+                  normalizePath(resolvedFsPath || normalizedFsPath),
+              )
+              if (optimizedDep) {
+                return depsOptimizer.getOptimizedDepId(optimizedDep)
+              }
+            }
+
             if (depsOptimizer.isOptimizedDepFile(normalizedFsPath)) {
               // Optimized files could not yet exist in disk, resolve to the full path
               // Inject the current browserHash version if the path doesn't have one
diff --git a/playground/optimize-deps/__tests__/optimize-deps.spec.ts b/playground/optimize-deps/__tests__/optimize-deps.spec.ts
index d6febaafad2c17..5b16169b449072 100644
--- a/playground/optimize-deps/__tests__/optimize-deps.spec.ts
+++ b/playground/optimize-deps/__tests__/optimize-deps.spec.ts
@@ -126,6 +126,24 @@ test('forced include', async () => {
     .toMatch(`[success]`)
 })
 
+test.runIf(isServe)(
+  'optimizes a project source file imported relatively',
+  async () => {
+    await expect
+      .poll(() => page.textContent('.source-include'))
+      .toBe('source include')
+
+    const metadata = readDepOptimizationMetadata()
+    const optimizedFile = metadata.optimized['./source-include.js'].file
+    const response = await fetch(
+      new URL('/source-include-entry.js', viteTestUrl),
+    )
+    const source = await response.text()
+
+    expect(source).toContain(`/node_modules/.vite/deps/${optimizedFile}`)
+  },
+)
+
 test('import * from optimized dep', async () => {
   await expect.poll(() => page.textContent('.import-star')).toMatch(`[success]`)
 })
diff --git a/playground/optimize-deps/index.html b/playground/optimize-deps/index.html
index 2e3b6607dabff7..dfeab677720d70 100644
--- a/playground/optimize-deps/index.html
+++ b/playground/optimize-deps/index.html
@@ -58,6 +58,10 @@ <h2>Detecting linked src package and optimizing its deps (lodash-es)</h2>
 <h2>Optimizing force included dep even when it's linked</h2>
 <div class="force-include"></div>
 
+<h2>Optimizing a project source file through a relative import</h2>
+<div class="source-include"></div>
+<script type="module" src="./source-include-entry.js"></script>
+
 <h2>Dep with CSS</h2>
 <div class="dep-linked-include">This should be red</div>
 
diff --git a/playground/optimize-deps/source-include-entry.js b/playground/optimize-deps/source-include-entry.js
new file mode 100644
index 00000000000000..b6131c9c2d0289
--- /dev/null
+++ b/playground/optimize-deps/source-include-entry.js
@@ -0,0 +1,3 @@
+import { message } from './source-include'
+
+document.querySelector('.source-include').textContent = message
diff --git a/playground/optimize-deps/source-include.js b/playground/optimize-deps/source-include.js
new file mode 100644
index 00000000000000..8049e851090730
--- /dev/null
+++ b/playground/optimize-deps/source-include.js
@@ -0,0 +1 @@
+export const message = 'source include'
diff --git a/playground/optimize-deps/vite.config.js b/playground/optimize-deps/vite.config.js
index 3a1abe05c6a50e..d082c7bd59ed7f 100644
--- a/playground/optimize-deps/vite.config.js
+++ b/playground/optimize-deps/vite.config.js
@@ -30,6 +30,7 @@ export default defineConfig({
       // only referenced by the import that injectImportIntoOptimizedDep()
       // adds at serve time, so the scanner can never discover it
       '@vitejs/test-dep-cjs-for-injected-import',
+      './source-include.js',
     ],
     exclude: [
       '@vitejs/test-nested-exclude',