diff --git a/topics/genome-annotation/tutorials/braker3/tutorial.md b/topics/genome-annotation/tutorials/braker3/tutorial.md
index 39c49710c534e0..ea6e0ca175da77 100644
--- a/topics/genome-annotation/tutorials/braker3/tutorial.md
+++ b/topics/genome-annotation/tutorials/braker3/tutorial.md
@@ -36,12 +36,16 @@ contributions:
authorship:
- rlibouba
- abretaud
+ editing:
+ - tflowers15
reviewing:
- deeptivarshney
- shiltemann
-
funding:
- eurosciencegateway
+ - unimelb
+ - melbournebioinformatics
+ - AustralianBioCommons
requirements:
- type: internal
@@ -85,9 +89,7 @@ To annotate our genome using Braker3, we will use the following files:
>
> {% snippet faqs/galaxy/histories_create_new.md %}
>
-> 2. Import the files from [Zenodo]({{ page.zenodo_link }}) or from
-> the shared data library (`GTN - Material` -> `{{ page.topic_name }}`
-> -> `{{ page.title }}`):
+> 2. Import the files from [Zenodo]({{ page.zenodo_link }}):
>
> ```
> https://zenodo.org/records/14770765/files/genome.fasta
@@ -97,7 +99,6 @@ To annotate our genome using Braker3, we will use the following files:
>
> {% snippet faqs/galaxy/datasets_import_via_link.md %}
>
-> {% snippet faqs/galaxy/datasets_import_from_data_library.md %}
>
{: .hands_on}
@@ -120,7 +121,7 @@ Here's how to run STAR with this specific parameter (**"Read alignement tags to
> - {% icon param-file %} *"RNA-Seq FASTQ/FASTA file, forward reads"*: `rnaseq_R1.fq.gz` (Input dataset)
> - {% icon param-file %} *"RNA-Seq FASTQ/FASTA file, reverse reads"*: `rnaseq_R2.fq.gz` (Input dataset)
> - *"Custom or built-in reference genome"*: `Use reference genome from history and create temporary index`
-> - {% icon param-file %} *"Select a reference genome"*: `genome_masked.fasta` (Input dataset)
+> - {% icon param-file %} *"Select a reference genome"*: `genome.fasta` (Input dataset)
> - *"Length of the SA pre-indexing string"*: `11`
> - In *"BAM output format specification"*:
> - In *"Read alignement tags to include in the BAM output"*:
@@ -132,9 +133,9 @@ Here's how to run STAR with this specific parameter (**"Read alignement tags to
We now have the following required inputs:
-- A masked genome in FASTA format
-- RNAseq sequence alignments in BAM format
-- Protein annotation in FASTA format
+- A masked genome in FASTA format: `genome.fasta`
+- RNAseq sequence alignments in BAM format: `RNASeq.bam`
+- Protein annotation in FASTA format: `protein_sequences.fasta`
With these files, We can run [**Braker3**](https://github.com/Gaius-Augustus/BRAKER) to perform the structural annotation of the genome.
@@ -142,10 +143,10 @@ With these files, We can run [**Braker3**](https://github.com/Gaius-Augustus/BRA
> Genome annotation with Braker3
>
> 1. {% tool [Braker3](toolshed.g2.bx.psu.edu/repos/iuc/braker3/braker3/3.0.8+galaxy0) %} with the following parameters:
-> - {% icon param-file %} *"Assembly to annotate"*: `genome_masked.fasta` (Input dataset)
+> - {% icon param-file %} *"Assembly to annotate"*: `genome.fasta` (Input dataset)
> - *"Species name"*: `Mucor mucedo`
-> - {% icon param-file %} *"RNA-seq mapped to genome to train Augustus/GeneMark"*: `rnaseq.bam` (Input dataset)
-> - {% icon param-file %} *"Proteins to map to genome"*: `SwissProt_subset.fasta` (Input dataset)
+> - {% icon param-file %} *"RNA-seq mapped to genome to train Augustus/GeneMark"*: `RNASeq.bam` (Input dataset)
+> - {% icon param-file %} *"Proteins to map to genome"*: `protein_sequences.fasta` (Input dataset)
> - {% icon param-file %} *"Fungal genome"*: select `Yes`
> - *"Output format"*: `GFF3`
>
@@ -182,8 +183,8 @@ So first generate these sequences:
> 1. {% tool [GFFread](toolshed.g2.bx.psu.edu/repos/devteam/gffread/gffread/2.2.1.4+galaxy0) %} with the following parameters:
> - {% icon param-file %} *"Input GFF3 or GTF feature file"*: output of {% tool [Braker](toolshed.g2.bx.psu.edu/repos/iuc/braker3/braker3/3.0.8+galaxy0) %}
> - In *"Reference Genome"* select: `From your history` (Input dataset)
-> - *"Genome Reference Fasta"*: `masked genome` (Input dataset)
-> - In *"Select fasta outputs"* select: `fasta file with spliced exons for each GFF transcript (-y)`
+> - *"Genome Reference Fasta"*: `genome.fasta` (Input dataset)
+> - In *"Select fasta outputs"* select: `protein fasta file with the translation of CDS for each record (-y)`
> - *"full GFF attribute preservation (all attributes are shown)"*: `Yes`
> - *"decode url encoded characters within attributes"*: `Yes`
> - *"warn about duplicate transcript IDs and other potential problems with the given GFF/GTF records"*: `Yes`
@@ -218,7 +219,7 @@ The parameters for running BUSCO on the masked genome:
> BUSCO in genome mode
>
> 1. {% tool [Busco](toolshed.g2.bx.psu.edu/repos/iuc/busco/busco/5.7.1+galaxy0) %} with the following parameters:
-> - {% icon param-file %} *"Sequences to analyse"*: `masked genome` (Input dataset)
+> - {% icon param-file %} *"Sequences to analyse"*: `genome.fasta` (Input dataset)
> - *"Mode"*: `Genome assemblies (DNA)`
> - *"Auto-detect or select lineage?"*: `Select lineage`
> - *"Lineage"*: `Mucorales`
@@ -280,7 +281,7 @@ This browser enables you to navigate along the chromosomes of the genome and vie
>
> 1. {% tool [JBrowse](toolshed.g2.bx.psu.edu/repos/iuc/jbrowse/jbrowse/1.16.11+galaxy1) %} with the following parameters:
> - *"Reference genome to display"*: `Use a genome from history`
-> - {% icon param-file %} *"Select the reference genome"*: `genome_masked.fasta` (Input dataset)
+> - {% icon param-file %} *"Select the reference genome"*: `genome.fasta` (Input dataset)
> - In *"Track Group"*:
> - {% icon param-repeat %} *"Insert Track Group"*
> - *"Track Category"*: `Annotation`