diff --git a/benchmarks/benchmarks/traj_reader.py b/benchmarks/benchmarks/traj_reader.py
index 3a203a3707..3e583c9824 100644
--- a/benchmarks/benchmarks/traj_reader.py
+++ b/benchmarks/benchmarks/traj_reader.py
@@ -28,19 +28,26 @@
 except ImportError:
     pass
 
+try:
+    from MDAnalysis.coordinates.PDB import PDBReader
+    from MDAnalysisTests.datafiles import PDB_multiframe
+except ImportError:
+    pass
+
 traj_dict = {
     "XTC": [XTC, XTCReader],
     "TRR": [TRR, TRRReader],
     "DCD": [DCD, DCDReader],
     "NCDF": [NCDF, NCDFReader],
     "TRC": [TRC_TRAJ_SOLV, TRCReader],
+    "PDB": [PDB_multiframe, PDBReader],
 }
 
 
 class TrajReaderCreation(object):
     """Benchmarks for trajectory file format reading."""
 
-    params = ["XTC", "TRR", "DCD", "NCDF", "TRC"]
+    params = ["XTC", "TRR", "DCD", "NCDF", "TRC", "PDB"]
     param_names = ["traj_format"]
 
     def setup(self, traj_format):
@@ -57,7 +64,7 @@ def time_reads(self, traj_format):
 class TrajReaderIteration(object):
     """Benchmarks for trajectory file format striding."""
 
-    params = ["XTC", "TRR", "DCD", "NCDF", "TRC"]
+    params = ["XTC", "TRR", "DCD", "NCDF", "TRC", "PDB"]
     param_names = ["traj_format"]
 
     def setup(self, traj_format):
diff --git a/package/CHANGELOG b/package/CHANGELOG
index 93ef2d158e..b3ccf32241 100644
--- a/package/CHANGELOG
+++ b/package/CHANGELOG
@@ -60,6 +60,7 @@ Fixes
    DSSP by porting upstream PyDSSP 0.9.1 fix (Issue #4913)
 
 Enhancements
+ * Added ASV benchmark for PDB trajectory reading (PR #5394)
  * Read Linear Angle bonded interaction entries from GROMACS TPR files and 
    add the angles to the topology (Issue #5361, PR #5372)
  * Enables parallelization for analysis.atomicdistances.AtomicDistances